Training Materials

The WHO Collaborating Centre for Applied Biosafety Programmes and Research at the Division of Occupational Health and Safety, National Institutes of Health developed the 2nd Edition of the Instructor's Guide for Biosafety Training. The Guide is a teaching tool. Its purpose is twofold. The Guide is an instructor's resource for teaching essential laboratory biosafety principles and practices through lectures, visual presentations and interactive activities. Second, instructors can use this Guide to introduce and teach the discipline of biosafety to the next generation of researchers and other laboratory personnel.

The design and organization of the Guide will facilitate instruction and learning. Each lecture has an accompanying visual presentation (in PowerPoint format) that augments and reinforces the lecture material. The lectures include suggested references for further study by instructors and students. Some lectures include training tips and ideas for instructors to follow to enhance the teaching experience. In addition, the lecture pages provide dedicated space for instructor notes. The interactive activities include questions to help instructors stimulate active discussions among students in the classroom and laboratory setting.

The APBTN encourages you to download the Guide for instruction and education purposes. Feel free to incorporate new information references and visuals to:

  1. Enhance the quality of the course or learning experience.
  2. Meet the unique needs of institutions or countries
  3. Adapt the training materials to resources and infrastructure that are available in various locales.

You may also download the individual presentations and documents by selecting the topics listed below.

"AN INSTRUCTOR'S GUIDE FOR BIOSAFETY TRAINING"

LECTURE OUTLINES AND PRESENTATIONS - UNIT I

A. General Guidance for Instructors

Module 1 Training and Evaluation: Biosafety Training Techniques for the Adult Learner

Supplemental Material:

References
An Instructor's Guide for Biosafety Training - A Study Guide for the Second Edition - pdf
Biosafety Training Techniques for the Adult Learner - Word
Sample Course Evaluation Tool - pdf
Sample Knowledge Assessment Tool - Word

B. Recognizing and Assessing Biological Hazards in the Laboratory

Module 2 Laboratory-associated Infections

Supplemental Material:

Case Studies 1 and 6

Module 3 Aerosol Hazards in the Laboratory

Supplemental Material:

Case Studies 3 and 5
Laboratory Exercises 1 and 2

Module 4 Biosafety Principles

Supplemental Material:

Case Study 2

Module 5 Classification of Microorganisms by Risk Group

Supplemental Material:

Case Study 2
References
Canadian Laboratory Biosafety Guidelines - pdf
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories - pdf
Biosafety Levels and Risk Groups: A Review - ppt
Singapore Biosafety Guidelines for Research on Genetically Modified Organisms - pdf
U.S. NIH Guidelines for Research Involving Recombinant DNA Molecules - pdf
WHO Laboratory Biosafety Manual - pdf

Module 6 Microbiological Risk Assessment in the Laboratory

Supplemental Material:

Case Studies 1, 2, and 6
References
Canadian Laboratory Biosafety Guidelines - pdf
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories - pdf
WHO Laboratory Biosafety Manual - pdf

C. Biosafety Practices, Equipment and Facility Safeguards

Module 7 Laboratory Safeguards and Procedures

Supplemental Material:

Case Studies 2 and 3
Laboratory Exercises 1, 3, and 5
References
WHO Guidelines On Hand Hygiene in Health Care - pdf

Module 8 Minimizing Aerosols

Supplemental Material:

Case Study 3
Laboratory Exercises 1 and 6

Module 9 Biological Safety Cabinets

Supplemental Material:

Laboratory Exercises 2 and 6
References
CDC/NIH Primary Containment for Biohazards: Selection, Installation and Use of Biological Safety Cabinets - pdf

Module 10 Decontamination and Sterilization - Heat Treatments

Supplemental Material:

Laboratory Exercise 4

Module 11 Decontamination and Sterilization - Chemicals

Supplemental Material:

Laboratory Exercise 6
References
Additional Chemical Germicide Slides - ppt

Module 12 Emergency Procedures

Supplemental Material:

Case Study 3
Laboratory Exercise 7

Module 13 Post-work Safeguards and Procedures

Supplemental Material:

Laboratory Exercise 5

Module 14 Waste Disposal Procedures

Supplemental Material:

Laboratory Exercise 4

Module 15 Laboratory Design and Facilities

Supplemental Material:

Case Study 7
Laboratory Exercise 5
References
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories - pdf
Design Concept Drawing - pdf
Laboratory Design - ppt
WHO Laboratory Biosafety Manual - pdf


D. Additional Laboratory Hazards and Practices

Module 16 Laboratory Animal Handling Practices

Supplemental Material:

Case Studies 2, 4, and 5
References
Laboratory Animal Handling Practices - ppt
Table of Zoonotic Hazards - Word

Module 17 Biosafety Aspects of Transmissible Spongiform Encephalopathy Agents (TSEs) - Prions

Supplemental Material:

References
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories - pdf
WHO Laboratory Safety Manual - pdf

Module 18 Biosafety and Recombinant DNA Laboratories

Supplemental Material:

Case Study 2

Module 19 Handling and Storage of Hazardous Chemicals

E. Administration and Risk Management

Module 20 Elements of a Laboratory Biosafety Programmes

Supplemental Material:

References
Biosafety Program Management - ppt

Module 21 Biosafety Officer and Biosafety Committees

Module 22 Safety for Support Staff

F. Laboratory Biosecurity

Module 23 Laboratory Biosecurity Concepts

Supplemental Material:

References
WHO Biorisk management: Laboratory Biosecurity Guidance - pdf
Biosecurity - ppt

Module 24 Emergency Response and Security in the Laboratory

Supplemental Material:

Laboratory Exercise 7

G. Transport of Infectious Substances

Module 25 Safe Transport of Infectious Substances and Diagnostic Specimens

Supplemental Material:

References
Appendix A - Guidance Document: Infectious Substances International Civil Aviation Organization Technical
Instructions for the Safe Transport of Dangerous Goods by Air, 2005-2006 - pdf
Dangerous Goods List and Limited Quantities Exceptions - pdf
Division 6.2 Infectious Substance Shipping Guide: Classification, Packing, Marking and Labeling - pdf
Global TV News Clip 03-04-05 - mpg
Shipper Explosion Movie - avi
Shipping Information Summary - jpeg
UN Classifications - pdf

CASE STUDIES - UNIT II

Case Study 1 - Unknown Route of Transmission of Human Immunodeficiency Virus

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Supplemental to:

Module 2 - Laboratory-associated Infections
Module 6 - Microbiological Risk Assessment in the Laboratory

Case Study 2 - West Nile Pathogenesis (Issues A, B, and C)

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Supplemental to:

Module 4 - Biosafety Principles
Module 5 - Classification of Microorganisms by Risk Group
Module 6 - Microbiological Risk Assessment in the Laboratory
Module 7 - Laboratory Safeguards and Procedures
Module 16 - Laboratory Animal Handling Practices
Module 18 - Biosafety and Recombinant DNA Laboratories

Case Study 3 - Airborne Transmission of an Arenavirus Resulting from a Centrifuge Accident

Download Case Study

Supplemental to:

Module 3 - Aerosol Hazards in the Laboratory
Module 7 - Laboratory Safeguards and Procedures
Module 8 - Minimizing Aerosols
Module 12 - Emergency Procedures

Case Study 4 - Lymphocytic Choriomeningitis (LCM)

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Supplemental to:

Module 16 - Laboratory Animal Handling Practices

Case Study 5 - Coxiella burnetii

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Supplemental to:

Module 3 - Aerosol Hazards in the Laboratory
Module 16 - Laboratory Animal Handling Practices

Case Study 6 - Possible Laboratory-associated Infection with Influenzae

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Supplemental to:

Module 2 - Laboratory-associated Infections
Module 6 - Microbiological Risk Assessment in the Laboratory

Case Study 7 - Understanding the Principles of Laboratory Design

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Supplemental to:

Module 15 - Laboratory Design and Facilities

LABORATORY EXERCISES - UNIT III

Using the Laboratory Exercises

Laboratory Exercise 1 - Use of Pipetting Aids and Devices

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Supplemental to:

Module 3 - Aerosol Hazards in the Laboratory
Module 7 - Laboratory Safeguards and Procedures
Module 8 - Minimizing Aerosols

Laboratory Exercise 2 - Working in Class II Biological Safety Cabinets

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Supplemental to:

Module 3 - Aerosol Hazards in the Laboratory
Module 9 - Biological Safety Cabinets

Laboratory Exercise 3 - Streaking Plates, Flaming Wire Loops

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Supplemental to:

Module 7 - Laboratory Safeguards and Procedures

Laboratory Exercise 4 - Sterilization

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Supplemental to:

Module 10 - Decontamination and Sterilization - Heat Treatments
Module 14 - Waste Disposal Procedures

Laboratory Exercise 5 - Work Practices in BSL-3 Containment (Mock-up of BSL-3)

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Supplemental to:

Module 7 - Laboratory Safeguards and Procedures
Module 13 - Post-work Safeguards and Procedures
Module 15 - Laboratory Design and Facilities

Laboratory Exercise 6 - Biological Fluids and Inactivation of Chlorine by Organic Material

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Supplemental to:

Module 8 - Minimizing Aerosols
Module 9 - Biological Safety Cabinets
Module 11 - Decontamination and Sterilization - Chemicals

Laboratory Exercise 7 - Cleaning Up a Spill of an Infectious Substance in a Laboratory

Download Laboratory Exercise

Supplemental to:

Module 12 - Emergency Procedures
Module 24 - Emergency Response and Security in the Laboratory

SUPPLEMENTAL MATERIALS

Introduction

Forward - Word
Course Purpose and Design - Word
Contents - Word

Module 1

Supplemental Material:
References
An Instructor's Guide for Biosafety Training - A Study Guide for the Second Edition - pdf
Biosafety Training Techniques for the Adult Learner - Word
Sample Course Evaluation Tool - pdf
Sample Knowledge Assessment Tool - Word

Module 5

Supplemental Material:
References
Canadian Laboratory Biosafety Guidelines - pdf
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories - pdf
Biosafety Levels and Risk Groups: A Review - ppt
Singapore Biosafety Guidelines for Research on Genetically Modified Organisms - pdf
U.S. NIH Guidelines for Research Involving Recombinant DNA Molecules - pdf
WHO Laboratory Biosafety Manual - pdf

Module 11

Supplemental Material:
References
Additional Chemical Germicide Slides - ppt

Module 15

Supplemental Material:
References
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories - pdf
Design Concept Drawing - pdf
Laboratory Design - ppt
WHO Laboratory Biosafety Manual - pdf

Module 16

Supplemental Material:
References
Laboratory Animal Handling Practices - ppt
Table of Zoonotic Hazards - Word

Module 20

Supplemental Material:
References
Biosafety Program Management - ppt

Module 23

Supplemental Material:
References
WHO Biorisk management: Laboratory Biosecurity Guidance - pdf
Biosecurity - ppt

Module 25

Supplemental Material:
References
Appendix A - Guidance Document: Infectious Substances International Civil Aviation Organization Technical
Instructions for the Safe Transport of Dangerous Goods by Air, 2005-2006 - pdf
Dangerous Goods List and Limited Quantities Exceptions - pdf
Division 6.2 Infectious Substance Shipping Guide: Classification, Packing, Marking and Labeling - pdf
Global TV News Clip 03-04-05 - mpg
Shipper Explosion Movie - avi
Shipping Information Summary - jpeg
UN Classifications - pdf

Additional Publications

Canadian Laboratory Biosafety Guidelines - pdf
CDC/NIH Biosafety in Microbiological and Biomedical Laboratories - pdf
CDC/NIH Primary Containment for Biohazards - pdf
Singapore Biosafety Guidelines for Research on Genetically Modified Organisms - pdf
U.S. NIH Guidelines for Research Involving Recombinant DNA Molecules - pdf
WHO Guidelines on Hand Hygiene - pdf
WHO Laboratory Biosafety Manual (Chinese) - pdf
WHO Laboratory Biosafety Manual (English) - pdf
WHO Laboratory Biosafety Manual (French) - pdf
WHO Laboratory Biosafety Manual (Japanese) - pdf
WHO Laboratory Biosafety Manual (Spanish) - pdf
WHO Laboratory Biosafety Manual (Vietnamese) - pdf

Ancillary Materials

Binder Cover - Word
Binder Spine - Word
Disk 1 Cover - Word
Disk 2 Cover - Word
Tabs - Case Studies - pdf
Tabs - Exercises - pdf
Tabs - Units - Word